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Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome Editing.
Weber J, Valiante V, Nødvig CS, Mattern DJ, Slotkowski RA, Mortensen UH, Brakhage AA2017Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome Editing. ACS Synth Biol 6, 62-68.
Abstract
Filamentous fungi produce varieties of natural products even in a strain dependent manner. However, the genetic basis of chemical speciation between strains is still widely unknown. One example is trypacidin, a natural product of the opportunistic human pathogen Aspergillus fumigatus, which is not produced among different isolates. Combining computational analysis with targeted gene editing, we could link a single nucleotide insertion in the polyketide synthase of the trypacidin biosynthetic pathway and reconstitute its production in a nonproducing strain. Thus, we present a CRISPR/Cas9-based tool for advanced molecular genetic studies in filamentous fungi, exploiting selectable markers separated from the edited locus.
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Doctoral researchers
Dr. rer. nat. Jakob Weber
Discovery of secondary metabolites from Aspergillus fumigatus utilizing post-transcriptional histone modifications
Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute (HKI)
Molecular and Applied Microbiology
